On the date offollicle puncture a couple come together. The husband provides sperm. An essential condition is abstaining from sex for three days before the puncture. The sperm is prepared in a special way, the healthiest male gametes are chosen. Oocytes obtained in the course of follicle puncture are kept in special cups with nutrient medium, which are placed in an incubator, where the conditions, similar to those of the mother’s organism, are maintained (the same temperature, carbon content).
In 4-6 hours after the follicle puncture insemination is carried out: male gametes are added to oocytes. The male gametes attach to zona pellucida and secrete a special ferment, trying to penetrate inside with its help, but only one of several thousand of gametes will manage to do it and transmit its genetic material for a new life creation, that is for oocyte fertilization.